Fig. 3

Confirmation of exon-5-8 removal. The 5′ probe hybridized to XbaI-digested genomic DNA detects 5.6-kb WT bands, 4.2-kb CKO allele bands, and 7.9-kb KO allele bands. Tail DNA shown in the figure was isolated from pups generated by a female heterozygous for RelA ^CKO allele and hemizygous for Zp3-cre transgene, and a wild-type C57BL/6J male