Fig. 2

The effect of Shh on hedgehog signaling. a, b qRT-PCR and ELISA were used to demonstrate Shh expression levels after transfected with Shh viral plasmids. c Expression levels of Ptc, Smo and Gli1 were detected by western blotting. d Results were normalized to GAPDH protein expression. The representative results were from three independent experiments. Significant differences between the control group are indicated by * p < 0.05, **p < 0.01