Fig. 4
The effect of Shh on chondrogenesis after 21 days of differentiation induction in the RCCS environment. a Cellular morphology. b Toluidine blue staining on cell-microcarrier complexes. c Toluidine blue staining on the cells after inoculated onto slides. d Collagen II immunohistochemical staining on cell-microcarrier complexes. e Collagen II immune-histochemical staining on the cells after inoculated onto slides. Scale bar: 100 μm