Fig. 2

5-Aza-CdR treatment of CCL-22 cells reintroduces KPNA7 expression by demethylation of CpG sites in the proximal promoter of bovine KPNA7 gene. a Expression of KPNA7 was detected in 5-Aza-CdR treated CCL-22 cells by RT-PCR. b Methylation status of 11 CpG sites in the proximal promoter of bovine KPNA7 gene in the 5-Aza-CdR treated and control CCL-22 cells. Demethylation of the CpG sites in the treatment group, and hypermethylation of the CpG sites in the control group were observed. Three oocyte-specific hypomethylated sites (− 149, − 63 and − 54) are all methylated in the control group. Close circles indicate methylated sites; open circles indicate demethylated sites