Fig. 5

Effect of pharmacological Notch signaling inhibition and activation on mouse embryo developmental kinetics. Mouse 2.5 dpc embryos were in vitro cultured in the presence of a Notch inhibitor (DAPT) or Notch ligands Jagged1 and Jagged2 until 4.5 dpc. Embryos were observed after 24 h in culture (at 3.5 dpc; a), 36 h in culture (at 4.0 dpc; b) and 48 h in culture (at 4.5 dpc; c) and morphologically evaluated. In vitro culture of a subset of embryos was discontinued at 3.5 dpc or 4.0 dpc to perform transcription analysis. Numbers above bars indicate the number of viable embryos in culture / the number of total embryos. Different letters within the same developmental stage differ significantly, p < 0.05 (Chi-square test). Asterisks (*) indicate the exact p value of the Chi-square test