Fig. 3

Alizarin red staining of mineralized nodules. a Alizarin Red S coloration showed the mineralized nodules deposit in 4-10mMPi mediums under naked eye observation. b Alizarin Red S coloration demonstrated no mineralized nodules deposit in 2-10mMPi mediums without cells (2Pi(−), 4Pi(−),6Pi(−),8Pi(−),10Pi(−)) c Alizarin Red S coloration showed the mineralized nodules deposit in 4-10mMPi mediums under microscope(× 100)